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ECMM - ESCMID survey on goals and pratices of mycobiota characterization using NGS in European labs

mandatory answer

Question 1

Your Country :

Question 2

Your Lab city :

Question 3

Your Name :

Question 4

Your e.mail address :

Question 5

For you, the goals of using NGS to study the mycobiota are to (1 or more answers) :

Understand the pathophysiology of a dysbiosis

Contribute to a diagnosis

Implement a therapeutic strategy

Investigate epidemics

Evaluate the exposome (environmental studies)

Comments

Question 6

Are you using in routine practice NGS to characterize the mycobiota : Yes or No

Yes

uncheck

Comments

Question 7

If Yes, NGS is used in order to (1 or more answers):

Contribute to a diagnosis

Implement a therapeutic strategy

Investigate epidemics

evaluate the exposome

Comments

Question 8

Are you using NGS for experimental studies on mycobiota : Yes or No

Yes

uncheck

Comments

Question 9

If Yes, NGS is used in order to (1 or more answers) :

Contribute to a diagnosis

Implement a therapeutic strategy

Investigate epidemics

evaluate the exposome

Comments

Question 10

Which Mycobiota sites are you studying or plan to study (1 or more answers):

Upper respiratory tract

Lower respiratory tract

Digestive tract

Oral cavity

Skin

Environmental samples : please cite (air, water,dust, other...)in comments

Other :

Comments

Question 11

Regarding Lung, which diseases are concerned by analysis (1 or more answers) :

Cystic fibrosis

COPD

Asthma

Lung transplantation

Pneumonia

Other :

Comments

Question 12

Regarding digestive tract which diseases are concerned by your analysis (1 or more answers) :

Crohn disease

Chronic ulcerative colitis

Pseudomembranous colitis (Clostridium difficile colitis)

Other :

Comments

Question 13

Other systemic diseases :

Rheumatoid polyarthritis

Diabetis mellitus

AIDS

Other :

Comments

Question 14

Samples used for NGS processing during your studies (1 or more answers) :

Oral wash

Sputum

Broncho-alveolar lavage

Stool

Skin sample

Environmental sample : please cite (air, water, dust, other...) in comments

Comments

Question 15

Target used for mycobiota analysis (1 or more answers)

18S rRNA

ITS1

ITS2

28s rRNA

Shotgun approach

Comments

Question 16

Do you consider that bacterial microbiota must be associated when studying the mycobiota :

Yes

uncheck

Comments

Question 17

Do you consider that viral microbiota must be associated when studying the mycobiota :

Yes

uncheck

Comments

Question 18

Do you use propidium monoazide pretreatment :

Yes

uncheck

Comments

Question 19

Data analysis (1 or more answers) :

reads shorter then 150 bases are note analyzed

Genebank database used for alignments

CBS database used for alignments

Both Genebank and CBS databases used for alignments

Unite database used for alignments

Other databank :

Comments

Question 20

NGS machine used for sequencing (brand and model) :

Question 21

Are you interested in participating to an European study groupe : Yes or No
If yes :

On the lung site

On the digestive site

Other site, please cite :

Comments

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ECMM - ESCMID survey on goals and pratices of mycobiota characterization using NGS in European labs

Your Country :

Your Lab city :

Your Name :

Your e.mail address :

For you, the goals of using NGS to study the mycobiota are to (1 or more answers) :

Are you using in routine practice NGS to characterize the mycobiota : Yes or No

If Yes, NGS is used in order to (1 or more answers):

Are you using NGS for experimental studies on mycobiota : Yes or No

If Yes, NGS is used in order to (1 or more answers) :

Which Mycobiota sites are you studying or plan to study (1 or more answers):

Regarding Lung, which diseases are concerned by analysis (1 or more answers) :

Regarding digestive tract which diseases are concerned by your analysis (1 or more answers) :

Other systemic diseases :

Samples used for NGS processing during your studies (1 or more answers) :

Target used for mycobiota analysis (1 or more answers)

Do you consider that bacterial microbiota must be associated when studying the mycobiota :

Do you consider that viral microbiota must be associated when studying the mycobiota :

Do you use propidium monoazide pretreatment :

Data analysis (1 or more answers) :

NGS machine used for sequencing (brand and model) :

Are you interested in participating to an European study groupe : Yes or NoIf yes :

Are you interested in participating to an European study groupe : Yes or No
If yes :