ESCMID survey on the routine diagnosis and treatment of visceral leishmaniasis

Question 1

You work in :

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Question 2

Please specify your location : City and Country :

Question 3

There is a reference center for leishmaniasis in your country :

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Question 4

Your volume of analysis, please specify on an annual basis
- Number of suspected diagnosis of VL. Please specify :
- Number of positive diagnosis of VL. Please specify :

Question 5

Among these sample types, which one are possibly processed in your lab :

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Question 6

Among these samples types, which one are possibly processed in your lab for direct examination and/or culture :

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Question 7

Among these sample types, which one is recommended in first-line for the diagnosis of VL in your hospital :

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Question 8

Direct examination is :

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Question 9

Diagnosis Tools :
Direct examination is performed :

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Question 10

Culture of Leishmania is :

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Question 11

Culture is performed using (*supplemented with fetal calf serum +/- antibiotics) :

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Question 12

Serological tests are :

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Question 13

Serology is performed using :

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Question 14

Molecular detection is :

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Question 15

Molecular detection is performed using :

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Question 16

Targets of the primers for PCR available in your lab are :

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Question 17

Identification of Leishmania is performed using :

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Question 18

Targets for the sequencing available in your lab are :

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Question 19

Drugs available in your hospital for the treatment of VL are :

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Question 20

The first-line treatment in adults is :

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Question 21

The second-line treatment in adults is :

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Question 22

The first-line treatment in children is :

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Question 23

The second-line treatment in children is :

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Question 24

When using liposomal Amphotericin B (Ambisome), please specify the total dose and administration schedule for :

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Question 25

Do you perform in vitro sensitivity testing of Leishmania isolates in your lab :

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Question 26

Do you think that in vitro sensitivity testing of Leishmania would be helpful for the choice of the drug :

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Question 27

Do you use pharmacological drug monitoring in your hospital :

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Question 28

Do you think that pharmacological drug monitoring would be helpful for the choice of the drug :

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Question 29

At what term do you consider that the VL treatment succeeded of failed and on what sample :

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Question 30

The first-line treatment for secondary prophylaxis in adults is :

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Question 31

The first-line treatment for secondary prophylaxis in children is :

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Question 32

When using liposomal Amphotericin B (Ambisome) for secondary prophylaxis, please specify the dose and administration schedule :

Question 33

When using another drug for secondary prophylaxis, please specify the dose and administration schedule :