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ESGCP/ESCMID-LeishMan survey on the routine diagnosis and treatment of visceral leishmaniasis

The objective is to have a picture of the diagnostic process and the principles of treatment of Visceral leishmaniasis in your centre. The two last questions concern the diagnostic process of cutaneous/mucocutaneous leishmaniasis.

Question 1

You work in :

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Question 2

Please specify your location : City and Country :

Question 3

There is a reference center for leishmaniasis in your country :

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Question 4

Your volume of analysis, please specify on an annual basis
- Number of suspected diagnosis of Visceral leishmaniasis. Please specify:
- Number of positive diagnosis of Visceral leishmaniasis. Please specify:

Question 5

Among these samples types, wich one are possibly processed in your lab for direct examination and/or culture and/or PCR:

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Question 6

Among these sample types, wich one is recommended in first-line for the diagnosis of VL in your hospital :

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Question 7

Direct examination is :

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Question 8

Diagnosis Tools :
Direct examination is performed :

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Question 9

Culture of Leishmania is :

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Question 10

Culture is performed using (*supplemented with fetal calf serum +/- antibiotics) :

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Question 11

Serological tests are :

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Question 12

Serology is performed using :

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Question 13

Molecular detection is

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Question 14

Molecular detection is performed using :

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Question 15

Targets of the primers for PCR available in your lab are :

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Question 16

Identification of Leishmania is performed using :

Question 17

Targets for the sequencing available in your lab are :

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Question 18

Drugs available in your hospital for the treatment of VL are :

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Question 19

The first-line treatment in adults is :

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Question 20

The second-line treatment in adults is :

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Question 21

The first-line treatment in children is :

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Question 22

The second-line treatment in children is :

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Question 23

When using liposomal Amphotericin B (Ambisome), please specify the total dose and administration schedule for :

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Question 24

Do you perform in vitro sensitivity testing of Leishmania isolates in your lab :

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Question 25

Do you think that in vitro sensitivity testing of Leishmania would be helpful for the choice of the drug :

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Question 26

Do you use pharmacological drug monitoring in your hospital :

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Question 27

Do you think that phamacological drug monitoring would be helpful for the choice of the drug :

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Question 28

At what term do you consider that the VL treatment succeeded or failed and on what sample :

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Question 29

The first-line treatment for secondary prophylaxis in adults is :

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Question 30

The first-line treatment for secondary prophylaxis in children is :

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Question 31

When using liposomal Amphotericin B (Ambisome) for secondary prophylaxis, please specify the dose and administration schedule :

Question 32

When using another drug of secondary prophylaxis, please specify the dose and administration schedule :

Question 33

Thank you for your answers on VL, two last questions regarding cutaneous/mucocutaneous leishmaniasis (CL/MCL) diagnosis in your lab.

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Question 34

Your volume of analysis, please specify on an annual basis
- Number of suspected diagnosis of CL/MCL. Please specify:
- Number of positive diagnosis of CL/MCL. Please specify:
- Number of positive diagnosis of PKDL. Please specify:




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